1 Scope
This standard specifies the examination method for Salmonella in foods.
This standard is applicable to the examination of salmonellae in foods.
2 Apparatus and Materials
In addition to the apparatuses for conventional sterilization and cultivation in microbiological laboratory, other apparatuses and materials are as follows:
2.1 Refrigerator: 2℃ ~ 5℃.
2.2 Constant temperature incubator: 36℃ ± 1℃, 42℃ ± 1℃.
2.3 Homogenizer.
2.4 Oscillator.
2.5 Electronic balance: sensibility of 0.1g.
2.6 Sterile conical flask: capacity of 500mL and 250mL.
2.7 Sterile pipette: 1mL (scale division of 0.01mL), 10mL (scale division of 0.1mL) or micropipette and pipette tip.
2.8 Sterile culture dish: diameter of 60 mm and 90mm.
2.9 Sterile test tube: 3mm×50mm, 10mm×75mm.
2.10 pH meter or pH colorimetric tube or precise pH paper.
2.11 Full-automatic microbe biochemical identification system.
2.12 Sterile capillary.
3 Culture Media and Reagents
3.1 Buffered peptone water (BPW): see A.1.
3.2 Tetrathionate broth (TTB): see A.2.
3.3 Selenite cystine (SC) broth: see A.3.
Contents
Foreword i
1 Scope
2 Apparatus and Materials
3 Culture Media and Reagents
4 Examination Procedure
5 Operation Procedure
6 Result and Report
Appendix A Culture Media and Reagents
Appendix B Common Salmonella Antigens
1 Scope
This standard specifies the examination method for Salmonella in foods.
This standard is applicable to the examination of salmonellae in foods.
2 Apparatus and Materials
In addition to the apparatuses for conventional sterilization and cultivation in microbiological laboratory, other apparatuses and materials are as follows:
2.1 Refrigerator: 2℃ ~ 5℃.
2.2 Constant temperature incubator: 36℃ ± 1℃, 42℃ ± 1℃.
2.3 Homogenizer.
2.4 Oscillator.
2.5 Electronic balance: sensibility of 0.1g.
2.6 Sterile conical flask: capacity of 500mL and 250mL.
2.7 Sterile pipette: 1mL (scale division of 0.01mL), 10mL (scale division of 0.1mL) or micropipette and pipette tip.
2.8 Sterile culture dish: diameter of 60 mm and 90mm.
2.9 Sterile test tube: 3mm×50mm, 10mm×75mm.
2.10 pH meter or pH colorimetric tube or precise pH paper.
2.11 Full-automatic microbe biochemical identification system.
2.12 Sterile capillary.
3 Culture Media and Reagents
3.1 Buffered peptone water (BPW): see A.1.
3.2 Tetrathionate broth (TTB): see A.2.
3.3 Selenite cystine (SC) broth: see A.3.
Contents of GB 4789.4-2016
Contents
Foreword i
1 Scope
2 Apparatus and Materials
3 Culture Media and Reagents
4 Examination Procedure
5 Operation Procedure
6 Result and Report
Appendix A Culture Media and Reagents
Appendix B Common Salmonella Antigens