Standard No.:	GB 5009.299-2024
English Name:	National food safety standard-Determination of lactoferrin in food
Chinese Name:	食品安全国家标准食品 中乳铁蛋白的测定
Chinese Classification:	X09    Hygiene, safety and labor protection
Professional Classification:	GB    National Standard
Source Content Issued by:	NHC; SAMR
Issued on:	2024-2-8
Implemented on:	2024-8-8
Status:	valid
Target Language:	English
File Format:	PDF
Word Count:	4000 words
Translation Price(USD):	120.0
Delivery:	via email in 1 business day

GB 5009.299-2024 National food safety standard - Determination of lactoferrin in food 1 Scope This standard specifies the high performance liquid chromatography (HPLC) for the determination of lactoferrin in food. This standard is applicable to the determination of bovine lactoferrin in pasteurized milk, modified milk, milk beverages, modified milk powder, and milk-based formula foods for infant and young children. This standard is not applicable to the determination of bovine lactoferrin in flavored fermented milk and bean-based formula foods for infant and young children. 2 Principle The bovine lactoferrin in the specimen is extracted via phosphate buffer solution, enriched and purified by heparin affinity column, separated by reversed-phase high performance liquid chromatograph column, detected by ultraviolet light at 280 nm, and quantified with the external standard method. 3 Reagents and materials Unless otherwise stated, reagents used in this method are all analytically pure, and water is of Grade 1 as specified in GB/T 6682. 3.1 Reagents 3.1.1 Acetonitrile (CH3CN): Chromatographically pure. 3.1.2 Trifluoroacetic acid (CF3CO2H): Chromatographically pure. 3.1.3 Sodium chloride (NaCl). 3.1.4 Phosphoric acid (H3PO4). 3.1.5 Disodium hydrogen phosphate (Na2HPO4).   3.2 Preparation of reagents 3.2.1 Phosphate buffer solution I: Weigh 7.1 g of disodium hydrogen phosphate and 58.4 g of sodium chloride, add 900 mL of water to dissolve them, adjust the pH to 8.0 ± 0.2 with phosphoric acid, and then dilute it to 1,000 mL with water. Freshly prepare before use. 3.2.2 Phosphate buffer solution II: Weigh 28.4 g of disodium hydrogen phosphate, add 900 mL of water to dissolve it, adjust the pH to 7.5 ± 0.2 with phosphoric acid, and then dilute it to 1,000 mL with water. Freshly prepare before use.

1 Scope 2 Principle 3 Reagents and materials 4 Instruments and apparatus 5 Analytical procedures 6 Expression of analysis results 7 Precision 8 Others Annex A Liquid chromatogram of the bovine lactoferrin standard solution Annex B Method for verifying heparin affinity column