GB/T 20382-2025 Textiles - Determination of extractable allergenic, carcinogenic and other dyestuffs
WARNING - Persons using this document shall have practical experience in regular laboratory work. This document does not purport to address all possible safety problems. It is the responsibility of the user to take appropriate safety and health measures and to ensure compliance with any national regulatory conditions.
1 Scope
This document describes the determination method of extractable allergenic, carcinogenic and other dyestuffs in textiles.
This standard is applicable to printed and dyed textiles.
The detection of other extractable dyestuffs may refer to this document upon validation.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-2008, ISO 3696:1987, MOD)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
allergenic dyestuffs
dyestuffs that cause allergic skin reactions
3.2
carcinogenic dyestuffs
dyestuffs that are classified as carcinogenic substances
4 Principle
The extractable carcinogenic, allergenic and other dyestuffs (according to Annex B) in the specimen are extracted with pyridine-water at 100 °C. The extract is filtered through a membrane filter and then determined by liquid chromatography-tandem mass spectrometry (LC‑MS/MS) or liquid chromatography-mass spectrometry (LC‑MS), with quantification by the external standard method.
5 Reagents and materials
Unless otherwise specified, analytically-pure reagents shall be adopted.
5.1 Pyridine, CAS No. 110-86-1.
5.2 Acetonitrile, chromatographic grade, CAS No. 75-05-8.
5.3 Ammonium acetate, CAS No. 631-61-8.
5.4 Laboratory water, grade I water as specified in GB/T 6682.
5.5 Pyridine-water (1:1): mix 500 mL of pyridine (5.1) with 500 mL of water (5.4). Store the resulting solution in a brown glass bottle.
5.6 Standard solutions of allergenic, carcinogenic and other dyestuffs. Prepare as follows.
a) Single-component standard stock solution (200 mg/L)
Prepare a single-component dyestuff standard stock solution at a mass concentration of 200 mg/L using pyridine-water (1:1) (5.5) or another suitable solvent such as methanol. The highest purity grade commercially available should be selected as the reference standard/reference material.
Note 1: The single-component standard stock solution can be stored in a refrigerator at 0 °C to 8 °C and is valid for one year.
b) Mixed standard intermediate solution (2 mg/L)
Transfer 0.5 mL of each single-component standard stock solution (200 mg/L) [5.6a)] of the various dyestuffs into the same 50 mL volumetric flask, and dilute to the mark with pyridine-water (1:1) (5.5) to prepare a mixed standard intermediate solution with a mass concentration of 2 mg/L.
Note 2: The mixed standard intermediate solution can be stored in a refrigerator at 0 °C to 8 °C and is valid for three months.
c) Mixed standard working solution
Accurately transfer a certain volume of the mixed standard intermediate solution [5.6b)] and dilute with pyridine-water (1:1) (5.5) to prepare a series of mixed standard working solutions at at least five different concentrations.
Note 3: The mixed standard working solution can be stored in a refrigerator at 0 °C to 8 °C and is valid for one month. Prepare the mixed standard working solutions at appropriate concentrations as needed. Due to reasons such as dyestuff purity (Basic Violet 3 may contain Basic Violet 1), the mixed standard working solutions are only used for screening. If any dyestuff is detected, single-component standard working solutions shall be prepared for quantification.
5.7 Polytetrafluoroethylene (PTFE) filter membrane, with a pore size of 0.22 μm.
6 Apparatus
6.1 Equipment and auxiliary equipment for sample processing
6.1.1 Routine laboratory equipment
6.1.2 Analytical balance, with readability of 0.001 g and 0.0001 g
6.1.3 Extractor, 20 mL to 40 mL, tubular hard glass extractor with a screw cap (with PTFE gasket)
6.1.4 Heating source, capable of heating to (100 ± 2) °C (heating block or laboratory sand bath, temperature‑controlled).
6.1.5 Autosampler vials with sealed caps.
6.2 Chromatographic apparatus (select one of the following)
6.2.1 Liquid chromatograph‑mass spectrometer (LC‑MS):
- Liquid chromatograph (LC);
- Electrospray ionization source (ESI);
- Mass spectrometer (MS);
- Chromatographic column.
6.2.2 Liquid chromatograph‑tandem mass spectrometer (LC‑MS/MS):
- Liquid chromatograph (LC);
- Electrospray ionization source (ESI);
- Tandem mass spectrometer (MS/MS);
- Chromatographic column.
7 Test procedure
7.1 Preparation of test specimen
Take a representative sample, cut it into small pieces of approximately 5 mm × 5 mm, and mix well. From these, weigh approximately 0.5 g (accurate to 0.001 g) as the test specimen.
Standard
GB/T 20382-2025 Textiles - Determination of extractable allergenic;carcinogenic and other dyestuffs (English Version)
Standard No.
GB/T 20382-2025
Status
to be valid
Language
English
File Format
PDF
Word Count
11000 words
Price(USD)
330.0
Implemented on
2027-11-1
Delivery
via email in 1~3 business day
Detail of GB/T 20382-2025
Standard No.
GB/T 20382-2025
English Name
Textiles - Determination of extractable allergenic;carcinogenic and other dyestuffs
GB/T 20382-2025 Textiles - Determination of extractable allergenic, carcinogenic and other dyestuffs
WARNING - Persons using this document shall have practical experience in regular laboratory work. This document does not purport to address all possible safety problems. It is the responsibility of the user to take appropriate safety and health measures and to ensure compliance with any national regulatory conditions.
1 Scope
This document describes the determination method of extractable allergenic, carcinogenic and other dyestuffs in textiles.
This standard is applicable to printed and dyed textiles.
The detection of other extractable dyestuffs may refer to this document upon validation.
2 Normative references
The following normative documents contain provisions which, through reference in this text, constitute provisions of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682 Water for analytical laboratory use - Specification and test methods (GB/T 6682-2008, ISO 3696:1987, MOD)
3 Terms and definitions
For the purposes of this document, the following terms and definitions apply.
3.1
allergenic dyestuffs
dyestuffs that cause allergic skin reactions
3.2
carcinogenic dyestuffs
dyestuffs that are classified as carcinogenic substances
4 Principle
The extractable carcinogenic, allergenic and other dyestuffs (according to Annex B) in the specimen are extracted with pyridine-water at 100 °C. The extract is filtered through a membrane filter and then determined by liquid chromatography-tandem mass spectrometry (LC‑MS/MS) or liquid chromatography-mass spectrometry (LC‑MS), with quantification by the external standard method.
5 Reagents and materials
Unless otherwise specified, analytically-pure reagents shall be adopted.
5.1 Pyridine, CAS No. 110-86-1.
5.2 Acetonitrile, chromatographic grade, CAS No. 75-05-8.
5.3 Ammonium acetate, CAS No. 631-61-8.
5.4 Laboratory water, grade I water as specified in GB/T 6682.
5.5 Pyridine-water (1:1): mix 500 mL of pyridine (5.1) with 500 mL of water (5.4). Store the resulting solution in a brown glass bottle.
5.6 Standard solutions of allergenic, carcinogenic and other dyestuffs. Prepare as follows.
a) Single-component standard stock solution (200 mg/L)
Prepare a single-component dyestuff standard stock solution at a mass concentration of 200 mg/L using pyridine-water (1:1) (5.5) or another suitable solvent such as methanol. The highest purity grade commercially available should be selected as the reference standard/reference material.
Note 1: The single-component standard stock solution can be stored in a refrigerator at 0 °C to 8 °C and is valid for one year.
b) Mixed standard intermediate solution (2 mg/L)
Transfer 0.5 mL of each single-component standard stock solution (200 mg/L) [5.6a)] of the various dyestuffs into the same 50 mL volumetric flask, and dilute to the mark with pyridine-water (1:1) (5.5) to prepare a mixed standard intermediate solution with a mass concentration of 2 mg/L.
Note 2: The mixed standard intermediate solution can be stored in a refrigerator at 0 °C to 8 °C and is valid for three months.
c) Mixed standard working solution
Accurately transfer a certain volume of the mixed standard intermediate solution [5.6b)] and dilute with pyridine-water (1:1) (5.5) to prepare a series of mixed standard working solutions at at least five different concentrations.
Note 3: The mixed standard working solution can be stored in a refrigerator at 0 °C to 8 °C and is valid for one month. Prepare the mixed standard working solutions at appropriate concentrations as needed. Due to reasons such as dyestuff purity (Basic Violet 3 may contain Basic Violet 1), the mixed standard working solutions are only used for screening. If any dyestuff is detected, single-component standard working solutions shall be prepared for quantification.
5.7 Polytetrafluoroethylene (PTFE) filter membrane, with a pore size of 0.22 μm.
6 Apparatus
6.1 Equipment and auxiliary equipment for sample processing
6.1.1 Routine laboratory equipment
6.1.2 Analytical balance, with readability of 0.001 g and 0.0001 g
6.1.3 Extractor, 20 mL to 40 mL, tubular hard glass extractor with a screw cap (with PTFE gasket)
6.1.4 Heating source, capable of heating to (100 ± 2) °C (heating block or laboratory sand bath, temperature‑controlled).
6.1.5 Autosampler vials with sealed caps.
6.2 Chromatographic apparatus (select one of the following)
6.2.1 Liquid chromatograph‑mass spectrometer (LC‑MS):
- Liquid chromatograph (LC);
- Electrospray ionization source (ESI);
- Mass spectrometer (MS);
- Chromatographic column.
6.2.2 Liquid chromatograph‑tandem mass spectrometer (LC‑MS/MS):
- Liquid chromatograph (LC);
- Electrospray ionization source (ESI);
- Tandem mass spectrometer (MS/MS);
- Chromatographic column.
7 Test procedure
7.1 Preparation of test specimen
Take a representative sample, cut it into small pieces of approximately 5 mm × 5 mm, and mix well. From these, weigh approximately 0.5 g (accurate to 0.001 g) as the test specimen.
