GB 15193.5-2014 National food safety standard — Mammalian erythrocyte micronucleus test
1 Scope
This standard specifies the basic test methods and technical requirements for mammalian erythrocyte micronucleus test.
This standard is applicable to evaluate the genotoxic effects of test substances.
2 Terms and definitions
2.1
micronucleus
entire chromatid or acentric fragment or ring of a chromosome that remains in the cytoplasm after the regular entry of chromosomes into daughter cells to form the nucleus in the late stages of mitosis. At telophase, one or more regular subnuclei are formed, which are contained in the cytoplasm of the cell
2.2
centromere
the region where chromosomes connect with the spindle fibers during cell division to allow the daughter chromosomes to move to the daughter cell poles in an orderly manner
2.3
orthochromatic erythrocyte
mature erythrocytes, which lack ribosomes can be distinguished from immature polychromatic erythrocytes by selective ribosome dyes
2.4
polychromatic erythrocyte
immature erythrocytes, which are in the intermediate stage of development and still contain ribosomes, so they can be distinguished from mature orthochromatic erythrocytels by selective ribosome dyes.
2.5
total erythrocyte
the sum of orthochromatic and polychromatic erythrocytes
3 Test objective and principle
Mammalian erythrocyte micronucleus test is used to detect chromosome damage or mitotic device damage in mature erythrocytes caused by the analysis of animal bone marrow and/or peripheral erythrocytes, resulting in the formation of micronucleus containing delayed chromosome fragments or whole chromosomes. This condition usually occurs as a result of the action of a chromosome breaking agent. It is also possible that in response to spindle poisons, the main nucleus fails to form and is replaced by a set of micronucleus, which is slightly larger than the typical micronucleus.
4 Apparatus and reagents
4.1 Apparatus
Anatomical instruments, biological microscopes, slides, etc.
4.2 Reagents
Note: Unless otherwise specified, all the reagents used are analytical pure and all the water used is redistilled water.
Foreword I
1 Scope
2 Terms and definitions
3 Test objective and principle
4 Apparatus and reagents
5 Test methods
6 Data processing and result evaluation
7 Test report
8 Interpretation of test
Standard
GB 15193.5-2014 National Food Safety Standard - Mammalian Erythrocyte Micronucleus Test (English Version)
Standard No.
GB 15193.5-2014
Status
valid
Language
English
File Format
PDF
Word Count
3500 words
Price(USD)
90.0
Implemented on
2015-5-1
Delivery
via email in 1 business day
Detail of GB 15193.5-2014
Standard No.
GB 15193.5-2014
English Name
National Food Safety Standard - Mammalian Erythrocyte Micronucleus Test
GB 15193.5-2014 National food safety standard — Mammalian erythrocyte micronucleus test
1 Scope
This standard specifies the basic test methods and technical requirements for mammalian erythrocyte micronucleus test.
This standard is applicable to evaluate the genotoxic effects of test substances.
2 Terms and definitions
2.1
micronucleus
entire chromatid or acentric fragment or ring of a chromosome that remains in the cytoplasm after the regular entry of chromosomes into daughter cells to form the nucleus in the late stages of mitosis. At telophase, one or more regular subnuclei are formed, which are contained in the cytoplasm of the cell
2.2
centromere
the region where chromosomes connect with the spindle fibers during cell division to allow the daughter chromosomes to move to the daughter cell poles in an orderly manner
2.3
orthochromatic erythrocyte
mature erythrocytes, which lack ribosomes can be distinguished from immature polychromatic erythrocytes by selective ribosome dyes
2.4
polychromatic erythrocyte
immature erythrocytes, which are in the intermediate stage of development and still contain ribosomes, so they can be distinguished from mature orthochromatic erythrocytels by selective ribosome dyes.
2.5
total erythrocyte
the sum of orthochromatic and polychromatic erythrocytes
3 Test objective and principle
Mammalian erythrocyte micronucleus test is used to detect chromosome damage or mitotic device damage in mature erythrocytes caused by the analysis of animal bone marrow and/or peripheral erythrocytes, resulting in the formation of micronucleus containing delayed chromosome fragments or whole chromosomes. This condition usually occurs as a result of the action of a chromosome breaking agent. It is also possible that in response to spindle poisons, the main nucleus fails to form and is replaced by a set of micronucleus, which is slightly larger than the typical micronucleus.
4 Apparatus and reagents
4.1 Apparatus
Anatomical instruments, biological microscopes, slides, etc.
4.2 Reagents
Note: Unless otherwise specified, all the reagents used are analytical pure and all the water used is redistilled water.
Contents of GB 15193.5-2014
Foreword I
1 Scope
2 Terms and definitions
3 Test objective and principle
4 Apparatus and reagents
5 Test methods
6 Data processing and result evaluation
7 Test report
8 Interpretation of test
