GB 4789.9-2025 National food safety standard - Food microbiological examination - Examination of Campylobacter jejuni and Campylobacter coli (English Version)
GB 4789.9-2025 National food safety standard -Food microbiological examination - Examination of Campylobacter jejuni and Campylobacter coli
1 Scope
This standard specifies the test methods for Campylobacter jejuni and Campylobacter coli in food.
This standard is applicable to the examination of Campylobacter jejuni and Campylobacter coli in food.
2 Equipment and materials
In addition to conventional sterilization, culture, and molecular detection equipment in microbiological laboratories, other equipment and materials are as follows.
2.1 Refrigerator: 2°C to 8°C and -18°C to -20°C.
2.2 Constant temperature incubator: 25°C±1°C, 36°C±1°C, 42℃±1°C.
2.3 Stomacher blender.
2.4 Sterile filter bag for stomacher.
2.5 Electronic balance: sensitivity of 0.1g, 0.01g, 0.001g.
2.6 Constant temperature water bath or metal bath device: 36°C to 100°C.
2.7 Oscillator.
2.8 Sterile petri dishes: diameter of 60 mm, 90 mm.
2.9 Sterile hydrophilic filter membrane: diameter of 47 mm, pore size of 0.45 μm.
2.10 Microaerobic culture device: providing microaerobic conditions (5% oxygen, 10% carbon dioxide, and 85% nitrogen).
2.11 Microscope: 10X to 1 000X.
2.12 Low-temperature high-speed centrifuge: 8 000g to 20 000g, 4°C.
2.13 pH meter or pH colorimetric tube or precision pH test paper.
2.14 Real-time fluorescence PCR instrument.
2.15 Real-time fluorescence PCR reaction tube.
2.16 Micropipette and tips: 0.1μL to 2.5μL, 0.5μL to 10μL, 2μL to 20μL, 20μL to 200μL, 100μL to 1 000μL.
2.17 Sterile centrifuge tubes: 50 mL, 100 mL, or larger capacity.
2.18 Screw-cap tube: 10 mL.
2.19 Sterile inoculation loop: 1μL, 10μL.
2.20 Sterile pipettes: 1 mL (with 0.01 mL graduations), 10 mL (with 0.1 mL graduations).
2.21 Microbial biochemical identification system.
2.22 Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS).
3 Culture media and reagents
3.1 0.1% peptone water: See A.1 in Annex A.
3.2 Sterile physiological saline: See A.2.
3.3 Campylobacter enrichment broth: See A.3.
3.4 Columbia blood agar: See A.4.
3.5 Karmali blood agar: See A.5.
3.6 Oxidase reagent: See A.6.
3.7 3% hydrogen peroxide (H2O2) solution: See A.7.
3.8 Indoxyl acetate strips: See A.8.
3.9 1 mol/L sodium thiosulfate (Na2S2O3) solution: See A.9.
3.10 Sodium hippurate hydrolysis reagents: See A.10.
3.11 Microbial biochemical identification kit or microbial biochemical identification system reagents.
3.12 Real-time fluorescence PCR detection kit or detection reagents (2× Real-time fluorescence PCR buffer, dNTPs, Taq DNA polymerase, and sterilized deionized water).
3.13 Campylobacter jejuni reference strain [NPRC(S)01.13897] or other equivalent strains.
3.14 Campylobacter coli reference strain [NPRC(S)01.13898] or other equivalent strains.
3.15 Campylobacter upsaliensis reference strain [NPRC(S)01.13899] or other equivalent strains.
3.16 Campylobacter lari reference strain [NPRC(S)01.13900] or other equivalent strains.
4 Examination procedures
Contents
Foreword i
1 Scope
2 Equipment and materials
3 Culture media and reagents
4 Examination procedures
5 Procedures
6 Results and report
Annex A Culture media and reagents
Standard
GB 4789.9-2025 National food safety standard - Food microbiological examination - Examination of Campylobacter jejuni and Campylobacter coli (English Version)
Standard No.
GB 4789.9-2025
Status
to be valid
Language
English
File Format
PDF
Word Count
8000 words
Price(USD)
240.0
Implemented on
2026-3-2
Delivery
via email in 1 business day
Detail of GB 4789.9-2025
Standard No.
GB 4789.9-2025
English Name
National food safety standard - Food microbiological examination - Examination of Campylobacter jejuni and Campylobacter coli
Chinese Name
食品安全国家标准 食品微生物学检验 空肠弯曲菌和结肠弯曲菌检验
Chinese Classification
Professional Classification
GB
ICS Classification
Issued by
National Health Commission and State Administration for Market Regulation
GB 4789.9-2025 National food safety standard -Food microbiological examination - Examination of Campylobacter jejuni and Campylobacter coli
1 Scope
This standard specifies the test methods for Campylobacter jejuni and Campylobacter coli in food.
This standard is applicable to the examination of Campylobacter jejuni and Campylobacter coli in food.
2 Equipment and materials
In addition to conventional sterilization, culture, and molecular detection equipment in microbiological laboratories, other equipment and materials are as follows.
2.1 Refrigerator: 2°C to 8°C and -18°C to -20°C.
2.2 Constant temperature incubator: 25°C±1°C, 36°C±1°C, 42℃±1°C.
2.3 Stomacher blender.
2.4 Sterile filter bag for stomacher.
2.5 Electronic balance: sensitivity of 0.1g, 0.01g, 0.001g.
2.6 Constant temperature water bath or metal bath device: 36°C to 100°C.
2.7 Oscillator.
2.8 Sterile petri dishes: diameter of 60 mm, 90 mm.
2.9 Sterile hydrophilic filter membrane: diameter of 47 mm, pore size of 0.45 μm.
2.10 Microaerobic culture device: providing microaerobic conditions (5% oxygen, 10% carbon dioxide, and 85% nitrogen).
2.11 Microscope: 10X to 1 000X.
2.12 Low-temperature high-speed centrifuge: 8 000g to 20 000g, 4°C.
2.13 pH meter or pH colorimetric tube or precision pH test paper.
2.14 Real-time fluorescence PCR instrument.
2.15 Real-time fluorescence PCR reaction tube.
2.16 Micropipette and tips: 0.1μL to 2.5μL, 0.5μL to 10μL, 2μL to 20μL, 20μL to 200μL, 100μL to 1 000μL.
2.17 Sterile centrifuge tubes: 50 mL, 100 mL, or larger capacity.
2.18 Screw-cap tube: 10 mL.
2.19 Sterile inoculation loop: 1μL, 10μL.
2.20 Sterile pipettes: 1 mL (with 0.01 mL graduations), 10 mL (with 0.1 mL graduations).
2.21 Microbial biochemical identification system.
2.22 Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS).
3 Culture media and reagents
3.1 0.1% peptone water: See A.1 in Annex A.
3.2 Sterile physiological saline: See A.2.
3.3 Campylobacter enrichment broth: See A.3.
3.4 Columbia blood agar: See A.4.
3.5 Karmali blood agar: See A.5.
3.6 Oxidase reagent: See A.6.
3.7 3% hydrogen peroxide (H2O2) solution: See A.7.
3.8 Indoxyl acetate strips: See A.8.
3.9 1 mol/L sodium thiosulfate (Na2S2O3) solution: See A.9.
3.10 Sodium hippurate hydrolysis reagents: See A.10.
3.11 Microbial biochemical identification kit or microbial biochemical identification system reagents.
3.12 Real-time fluorescence PCR detection kit or detection reagents (2× Real-time fluorescence PCR buffer, dNTPs, Taq DNA polymerase, and sterilized deionized water).
3.13 Campylobacter jejuni reference strain [NPRC(S)01.13897] or other equivalent strains.
3.14 Campylobacter coli reference strain [NPRC(S)01.13898] or other equivalent strains.
3.15 Campylobacter upsaliensis reference strain [NPRC(S)01.13899] or other equivalent strains.
3.16 Campylobacter lari reference strain [NPRC(S)01.13900] or other equivalent strains.
4 Examination procedures
Contents of GB 4789.9-2025
Contents
Foreword i
1 Scope
2 Equipment and materials
3 Culture media and reagents
4 Examination procedures
5 Procedures
6 Results and report
Annex A Culture media and reagents
