Standard No.:	GB/T 26635-2011
English Name:	Animal and vegetable fats and oils—Determination of tocopherol and tocotrienol contents by high-performance liquid chromatography
Chinese Name:	动植物油脂 生育酚及生育三烯酚含量测定 高效液相色谱法
Chinese Classification:	X14    Grease and oil processing and products
Professional Classification:	GB    National Standard
ICS Classification:	67.200.10 67.200.10    Animal and vegetable fats and oils 67.200.10
Source Content Issued by:	AQSIQ;SAC
Issued on:	2011-6-16
Implemented on:	2011-11-1
Status:	valid
Target Language:	English
File Format:	PDF
Word Count:	9000 words
Translation Price(USD):	220.0
Delivery:	via email in 1 business day

1 Scope This standard specifies a method for the determination of the contents of free α-, β-, γ-, and δ-tocopherols and tocotrienols (referred to jointly as tocols) in animal and vegetable fats and oils (referred to hereinafter as fats) by high-performance liquid chromatography (HPLC). For products containing tocopherol or tocotrienol esters, it is necessary to carry out a preliminary saponification. Note: a suitable method involving a cold saponification procedure is described in Annex B for information only. 2 Normative references The following normative documents contain provisions which, through reference in this standard, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections) to, or revisions of, any of these publications do not apply. However, parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the standards indicated below. For undated references, the latest edition applies. GB/T 15687 GB/T 15687 Animal and vegetable fats and oils - preparation of test sample (GB/T 15687-2008, ISO 661:2003, IDT) 3 Terms and definitions For the purpose of this standard, the following terms and definitions apply. 3.1 tocol content mass fraction of the individual tocols, determined using the method specified in this standard Note: the content is expressed in milligrams per kilogram as a whole number. 4 Principle

Foreword II 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Reagents 6 Apparatus 7 Sampling 8 Preparation of test sample 9 Procedure 10 Expression of results 11 Precision 12 Test report Annex A (Informative) Examples of chromatograms Annex B (Informative) Saponification Annex C (Informative) Results of interlaboratory tests Bibliography