Preface
This document is drafted in accordance with GB/T 1.1-2020 "Guidelines for Standardization Work, Part 1: Structure and Drafting Rules of Standardization Documents".
This document is the first part of GB/T 42239 Enzyme Preparation for Washing. The following parts have been published in GB/T 42239:
——Part 1: Alkaline protease
——Part 2: Lipase.
Please note that some contents of this document may involve patents. The issuing authority of this document does not bear the responsibility of identifying patents. This document is proposed by China Light Industry Federation.
This document is under the jurisdiction of the National Technical Committee for Standardization of Surfactants and Detergents (SAC/TC 272).
Introduction
The detergent industry is one of the important application fields of enzyme preparations. The formulation of GB/T42239 Enzyme Preparations for Detergent is of great significance for China's detergent industry to standardize the market of enzyme-containing detergent products, guide enterprises in the industry to innovate and develop new products, prevent bad enterprises from cheating consumers, and promote the rapid and healthy development of the detergent industry.
GB/T 42239 Enzyme Preparation for Washing is intended to consist of the following two parts:
——Part 1: Alkaline protease, which aims to establish the classification and technical requirements of alkaline protease for washing
——Part 2: Lipases, aiming to establish the classification and technical requirements of lipases for washing.
1 Scope
This document specifies the product classification, requirements, test methods, inspection rules, marking, packaging, transportation, storage and shelf life of alkaline protease for washing.
This document is applicable to the alkaline protease for detergent industry prepared by microbial fermentation, purification and other processes.
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references. For dated reference documents, only the version corresponding to that date is applicable to this document; For undated references, the latest version (including all amendments) is applicable to this document.
GB/T 191 Pictorial Marking of Packaging, Storage and Transportation
GB4789.2 National standard for food safety -- Microbiological examination of food -- Determination of total bacterial count
GB 4789.3 National standard for food safety -- Microbiological examination of food -- Coliform count
GB/T 6003.1 Test sieves - Technical requirements and tests - Part 1: Wire woven mesh test sieves
GB/T 6005 Test sieves - Basic dimensions of wire woven mesh perforating plates and electroformed sheets
GB/T 6368 Surface active agents - Determination of p value of aqueous solution - Potentiometric method
GB/T 6682 Water for analytical laboratory - Specification and test method
GB 15258 Rules for the Preparation of Chemical Safety Labels
JJF 1070 Rules for Metrological Inspection of Net Content of Products in Quantitative Packaging
QB/T 2739 Common test methods for washing products and preparation of test solution for titrimetric analysis (volumetric analysis)
3 Terms and definitions
The following terms and definitions apply to this document.
three point one
Alkali protease
It is an enzyme that can cut off the peptide bond inside the protein molecule under the alkaline reaction condition, and make the protein molecule become small molecule polypeptide and amino acid.
three point two
Alkali protease activity
The ability of protease to catalyze protein hydrolysis under alkaline conditions.
4 Product classification
It can be divided into liquid dosage form and solid dosage form according to product form.
5 Requirements
5.1 Appearance and physical and chemical performance requirements
The appearance and physical and chemical performance indicators of the product shall comply with the provisions of Table 1.
Table 1 Appearance and physical and chemical performance indexes
6 Test method
6.1 General rules
Unless otherwise specified, only reagents confirmed as analytically pure and grade III or above water specified in GB/T 6682 shall be used in the analysis.
6.2 Appearance
Take an appropriate amount of sample and place it in a dry and clean transparent experimental vessel for observation under non-direct light conditions.
6.3pH
Carry out according to the provisions of GB/T 6368.
7 Inspection rules
7.1 Inspection classification
7.1.1 Delivery inspection
The ex-factory inspection items are the appearance and enzyme activity specified in Table 1. When applicable to food detergent products, add the items specified in Table 2.
8 Marking, packaging, transportation, storage and shelf life
8.1 Signs
The outer packaging of the product shall be marked in accordance with the requirements of GB/T191.
The package of the product shall be firmly labeled. The label shall include the product name, net content, name, address and contact information of the manufacturer and/or distributor, production date and shelf life, storage conditions, batch number and other contents to be marked. The content of chemical safety label shall meet the requirements of GB 15258.
8.2 Packaging
The inner packaging of the product and/or the inner coating of the packaging container shall comply with relevant regulations.
8.3 Transportation
The product shall be handled with care during transportation, and shall not be upside down. It shall be strictly protected from rain and sun exposure. It is not allowed to step on and stack heavy objects on the box. The means of transport shall be clean, non-toxic and pollution-free. It shall not be mixed with toxic, harmful and corrosive substances.
8.4 Storage
The product should be stored in a cool and dry environment. It should not be stored with toxic, harmful and abrasive substances. Necessary protective measures shall be taken for stacking, and the stacking height shall be appropriate to avoid damage to the transportation package.
8.5 Shelf life
The product is under 25 ℃ and the shelf life is not less than 12 months,
Under the specified transportation, packaging and storage conditions, the enzyme activity within the shelf life shall not be lower than the marked enzyme activity. Note: The products beyond the shelf life still have use value, and the enzyme activity shall be re-measured during use.
Appendix A (normative) Determination of alkaline protease activity Fulin method (arbitration method)
Appendix B (informative) Determination of alkaline protease activity Ultraviolet spectrophotometry
Annex C (normative) Determination of particle size Sieve method
Forword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Product classification
5 Requirements
6 Test method
7 Inspection rules
8 Marking, packaging, transportation, storage and shelf life
Appendix A (normative) Determination of alkaline protease activity Fulin method (arbitration method)
Appendix B (informative) Determination of alkaline protease activity Ultraviolet spectrophotometry
Annex C (normative) Determination of particle size Sieve method
Preface
This document is drafted in accordance with GB/T 1.1-2020 "Guidelines for Standardization Work, Part 1: Structure and Drafting Rules of Standardization Documents".
This document is the first part of GB/T 42239 Enzyme Preparation for Washing. The following parts have been published in GB/T 42239:
——Part 1: Alkaline protease
——Part 2: Lipase.
Please note that some contents of this document may involve patents. The issuing authority of this document does not bear the responsibility of identifying patents. This document is proposed by China Light Industry Federation.
This document is under the jurisdiction of the National Technical Committee for Standardization of Surfactants and Detergents (SAC/TC 272).
Introduction
The detergent industry is one of the important application fields of enzyme preparations. The formulation of GB/T42239 Enzyme Preparations for Detergent is of great significance for China's detergent industry to standardize the market of enzyme-containing detergent products, guide enterprises in the industry to innovate and develop new products, prevent bad enterprises from cheating consumers, and promote the rapid and healthy development of the detergent industry.
GB/T 42239 Enzyme Preparation for Washing is intended to consist of the following two parts:
——Part 1: Alkaline protease, which aims to establish the classification and technical requirements of alkaline protease for washing
——Part 2: Lipases, aiming to establish the classification and technical requirements of lipases for washing.
1 Scope
This document specifies the product classification, requirements, test methods, inspection rules, marking, packaging, transportation, storage and shelf life of alkaline protease for washing.
This document is applicable to the alkaline protease for detergent industry prepared by microbial fermentation, purification and other processes.
2 Normative references
The contents of the following documents constitute essential provisions of this document through normative references. For dated reference documents, only the version corresponding to that date is applicable to this document; For undated references, the latest version (including all amendments) is applicable to this document.
GB/T 191 Pictorial Marking of Packaging, Storage and Transportation
GB4789.2 National standard for food safety -- Microbiological examination of food -- Determination of total bacterial count
GB 4789.3 National standard for food safety -- Microbiological examination of food -- Coliform count
GB/T 6003.1 Test sieves - Technical requirements and tests - Part 1: Wire woven mesh test sieves
GB/T 6005 Test sieves - Basic dimensions of wire woven mesh perforating plates and electroformed sheets
GB/T 6368 Surface active agents - Determination of p value of aqueous solution - Potentiometric method
GB/T 6682 Water for analytical laboratory - Specification and test method
GB 15258 Rules for the Preparation of Chemical Safety Labels
JJF 1070 Rules for Metrological Inspection of Net Content of Products in Quantitative Packaging
QB/T 2739 Common test methods for washing products and preparation of test solution for titrimetric analysis (volumetric analysis)
3 Terms and definitions
The following terms and definitions apply to this document.
three point one
Alkali protease
It is an enzyme that can cut off the peptide bond inside the protein molecule under the alkaline reaction condition, and make the protein molecule become small molecule polypeptide and amino acid.
three point two
Alkali protease activity
The ability of protease to catalyze protein hydrolysis under alkaline conditions.
4 Product classification
It can be divided into liquid dosage form and solid dosage form according to product form.
5 Requirements
5.1 Appearance and physical and chemical performance requirements
The appearance and physical and chemical performance indicators of the product shall comply with the provisions of Table 1.
Table 1 Appearance and physical and chemical performance indexes
6 Test method
6.1 General rules
Unless otherwise specified, only reagents confirmed as analytically pure and grade III or above water specified in GB/T 6682 shall be used in the analysis.
6.2 Appearance
Take an appropriate amount of sample and place it in a dry and clean transparent experimental vessel for observation under non-direct light conditions.
6.3pH
Carry out according to the provisions of GB/T 6368.
7 Inspection rules
7.1 Inspection classification
7.1.1 Delivery inspection
The ex-factory inspection items are the appearance and enzyme activity specified in Table 1. When applicable to food detergent products, add the items specified in Table 2.
8 Marking, packaging, transportation, storage and shelf life
8.1 Signs
The outer packaging of the product shall be marked in accordance with the requirements of GB/T191.
The package of the product shall be firmly labeled. The label shall include the product name, net content, name, address and contact information of the manufacturer and/or distributor, production date and shelf life, storage conditions, batch number and other contents to be marked. The content of chemical safety label shall meet the requirements of GB 15258.
8.2 Packaging
The inner packaging of the product and/or the inner coating of the packaging container shall comply with relevant regulations.
8.3 Transportation
The product shall be handled with care during transportation, and shall not be upside down. It shall be strictly protected from rain and sun exposure. It is not allowed to step on and stack heavy objects on the box. The means of transport shall be clean, non-toxic and pollution-free. It shall not be mixed with toxic, harmful and corrosive substances.
8.4 Storage
The product should be stored in a cool and dry environment. It should not be stored with toxic, harmful and abrasive substances. Necessary protective measures shall be taken for stacking, and the stacking height shall be appropriate to avoid damage to the transportation package.
8.5 Shelf life
The product is under 25 ℃ and the shelf life is not less than 12 months,
Under the specified transportation, packaging and storage conditions, the enzyme activity within the shelf life shall not be lower than the marked enzyme activity. Note: The products beyond the shelf life still have use value, and the enzyme activity shall be re-measured during use.
Appendix A (normative) Determination of alkaline protease activity Fulin method (arbitration method)
Appendix B (informative) Determination of alkaline protease activity Ultraviolet spectrophotometry
Annex C (normative) Determination of particle size Sieve method
Contents of GB/T 42239.1-2022
Forword
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Product classification
5 Requirements
6 Test method
7 Inspection rules
8 Marking, packaging, transportation, storage and shelf life
Appendix A (normative) Determination of alkaline protease activity Fulin method (arbitration method)
Appendix B (informative) Determination of alkaline protease activity Ultraviolet spectrophotometry
Annex C (normative) Determination of particle size Sieve method
