GB/T 46258-2025 Determination of hexamethylenetetramine in cosmetics—Liquid chromatography–tandem mass spectrometry English, Anglais, Englisch, Inglés, えいご
This is a draft translation for reference among interesting stakeholders. The finalized translation (passing thorugh draft translation, self-check, revision and varification) will be delivered upon being ordered.
ICS 71.100.70 CCS Y 42
National Standard of the People's Republic of China
GB/T 46258-2025
Determination of Hexamethylenetetramine in Cosmetics
Liquid Chromatography-Tandem Mass Spectrometry
Issued on: 2025-08-29
Implementation on: 2026-03-01
Issued by: State Administration for Market Regulation, Standardization Administration of China
Content
Preface
Introduction
1 Scope
2 Normative References
3 Terms and definitions
4 Principles
5 Reagents and Materials
6 Instruments and equipment
7 Sample Preparation and Testing Procedures
8 Experimental data processing
9 Recovery rate
10 Precision
11 Test Report
1 Scope
This document specifies the principle, reagents, instruments, sample preparation, experimental procedures, data processing, recovery rate, precision, and test reporting for the determination of hexamethylenetetramine in cosmetics by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Applicability: Aqueous solutions, creams, and emulsions. Method detection limit (MDL): 0.10 mg/kg. Method quantification limit (MQL): 0.20 mg/kg.
2 Normative References
GB/T 6682 Requirements for water in analytical laboratories (latest version).
3 Terms and Definitions
No terms require definition.
4 Principle
Sample preparation:
Vortex oscillation with 20% acetonitrile solution.
Ultrasonic extraction.
Liquid-liquid extraction purification using n-hexane.
Centrifugation and filtration.
Analysis:
LC-MS/MS separation with retention time and relative ion abundance for qualitative analysis.
Internal standard method for quantification.
5 Reagents and Materials
5.1 Water: GB/T 6682, Grade I.
5.2 Acetonitrile.
5.3 Ammonium acetate.
5.4 N-hexane.
5.5 20% Acetonitrile solution: 20 mL acetonitrile diluted to 100 mL with water.
5.6 Ammonium acetate solution (0.005 mol/L): 0.3854 g ammonium acetate dissolved in 1 L water.
Standard
GB/T 46258-2025 Determination of hexamethylenetetramine in cosmetics—Liquid chromatography–tandem mass spectrometry (English Version)
Standard No.
GB/T 46258-2025
Status
to be valid
Language
English
File Format
PDF
Word Count
6000 words
Price(USD)
180.0
Implemented on
2026-3-1
Delivery
via email in 1~3 business day
Detail of GB/T 46258-2025
Standard No.
GB/T 46258-2025
English Name
Determination of hexamethylenetetramine in cosmetics—Liquid chromatography–tandem mass spectrometry
GB/T 46258-2025 Determination of hexamethylenetetramine in cosmetics—Liquid chromatography–tandem mass spectrometry English, Anglais, Englisch, Inglés, えいご
This is a draft translation for reference among interesting stakeholders. The finalized translation (passing thorugh draft translation, self-check, revision and varification) will be delivered upon being ordered.
ICS 71.100.70 CCS Y 42
National Standard of the People's Republic of China
GB/T 46258-2025
Determination of Hexamethylenetetramine in Cosmetics
Liquid Chromatography-Tandem Mass Spectrometry
Issued on: 2025-08-29
Implementation on: 2026-03-01
Issued by: State Administration for Market Regulation, Standardization Administration of China
Content
Preface
Introduction
1 Scope
2 Normative References
3 Terms and definitions
4 Principles
5 Reagents and Materials
6 Instruments and equipment
7 Sample Preparation and Testing Procedures
8 Experimental data processing
9 Recovery rate
10 Precision
11 Test Report
1 Scope
This document specifies the principle, reagents, instruments, sample preparation, experimental procedures, data processing, recovery rate, precision, and test reporting for the determination of hexamethylenetetramine in cosmetics by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Applicability: Aqueous solutions, creams, and emulsions. Method detection limit (MDL): 0.10 mg/kg. Method quantification limit (MQL): 0.20 mg/kg.
2 Normative References
GB/T 6682 Requirements for water in analytical laboratories (latest version).
3 Terms and Definitions
No terms require definition.
4 Principle
Sample preparation:
Vortex oscillation with 20% acetonitrile solution.
Ultrasonic extraction.
Liquid-liquid extraction purification using n-hexane.
Centrifugation and filtration.
Analysis:
LC-MS/MS separation with retention time and relative ion abundance for qualitative analysis.
Internal standard method for quantification.
5 Reagents and Materials
5.1 Water: GB/T 6682, Grade I.
5.2 Acetonitrile.
5.3 Ammonium acetate.
5.4 N-hexane.
5.5 20% Acetonitrile solution: 20 mL acetonitrile diluted to 100 mL with water.
5.6 Ammonium acetate solution (0.005 mol/L): 0.3854 g ammonium acetate dissolved in 1 L water.
