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Standard
T/CSCB 0029-2024 Detection of Residual Pluripotent Stem Cells in Differentiated Cells―General requirements on TaqMan Quantitative PCR assay (English Version) |
Detail of T/CSCB 0029-2024
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Introduction of T/CSCB 0029-2024
本文件规定了分化细胞中残留多能干细胞检测的术语定义,一般要求,以及细胞样本的抽样方式和取样量、RNA提取、反转录、中间样本的保存与效期验证、检测靶标基因和内参基因的选择与对应引物和探针的设计、检测对照、定量PCR反应程序设计、结果判定、检测方法验证中的相关要求。
本标准适用于多能干细胞分化细胞中残留多能干细胞检测TaqMan探针实时荧光定量PCR方法的开发与验证。
Contents of T/CSCB 0029-2024