2.1.3 Identification test of air disinfection efficacy
2.1.3.1 Purpose
Detect the killing and/or cleaning effect of disinfecting apparatus and disinfectant on bacteria in the air in order to verify its disinfection efficacy to air. For the purposes of the disinfection efficacy of other methods to air, the relevant principles of this test may also be referred to.
2.1.3.2 Test equipment and apparatus
(1) The test bacterium is Staphylococcus albus 8032, and see 2.1.1.2 for the preparation method of the bacterial suspension.
(2) Sampling liquid [used for sampling with liquid-impacting sampler. During sterilization test using non-chemical factor, nutrient broth medium containing anti-foaming agent (octanol or olive oil) shall be used; during sterilization test using disinfectant, nutrient broth medium containing corresponding neutralizer (octanol or olive oil also added) shall be used].
(3) Neutralizer (qualified upon identification according to the method specified in 2.1.1.5).
(4) Phosphate buffer (PBS, 0.03 mol/L, pH 7.2).
(5) Common nutrient broth medium.
(6) Common nutrient agar medium. In the sterilization test using disinfectant, corresponding neutralizer need to be added in it.
(7) A pair of adjacent aerosol cabinets or chambers, one for disinfection test and the other for test control. The environment in which a pair of adjacent aerosol cabinets or chambers are located shall be the same (including temperature, humidity, lighting, airtightness and ventilation conditions). Cabinets (or chambers) should be constructed of aluminum alloy and glass. Temperature and humidity regulating devices, fan-filtering sterilization or other disinfection devices together with corresponding pipelines shall be installed. In addition, windows for spraying for bacteria contamination, operating sleeves for disinfectant supplying and sampling and transferring sample shall be opened.
(8) Spraying device for bacteria contamination, including air compressor, pressure gauge, gas flowmeter, aerosol sprayer, etc. More than 90% of bacterial aerosol particles shall have a diameter of 1μm~10μm.
(9) Air microorganism sampling device, including six-stage mesh air-impacting sampler, liquid-impacting sampler, air extraction equipment, gas flowmeter, etc.
(10) Environmental monitoring equipment, such as thermometers and hygrometers.
2.1.3.3 Test stage
Air disinfection tests are divided into laboratory tests, simulated field tests and field tests. The characteristics of the tests in three stages are shown in Table 2-5.
2.1.3.4 Operating procedures for laboratory tests and simulated field tests
(1) Take the bacterial suspension of test bacteria, filter it with sterile absorbent cotton, and dilute it with nutrient broth medium to the required concentration.
(2) Simultaneously adjust the temperature and relative humidity of the two aerosol cabinets (or chambers) to the required ones for the test.
(3) Put the equipment used into the aerosol cabinets (or chambers) at one time and close the doors. After that, all operations and operation of instruments and equipment are carried out outside the cabinets (or chambers) through windows with sealed sleeves or through remote controllers. The door may not be opened until the test is finished.
(4) Spray for bacteria contamination according to the set pressure, gas flow and spray time. Spray the contaminated bacteria while stirring with a fan. After the completion of spraying contaminated bacteria, continue to stir for 5min, and then let stand for 5min.
(5) At the same time, conduct sampling for the aerosol cabinets (chambers) of the control group and the test group before disinfection, respectively, as positive controls (i.e. the amount of contaminated bacteria) before conducting the test of the control group and the disinfection treatment of the test group. As for the air in the aerosol cabinets (chambers), the number of bacteria used as positive control shall reach 5´104cfu/m3~5´106cfu/m3.
Table 2-5 Characteristics of air disinfection tests at each stage
Standard
XDJSGF2022 Technical Specification for Disinfection, 2002 (English Version)
Standard No.
XDJSGF2022
Status
valid
Language
English
File Format
PDF
Word Count
130,000 words
Price(USD)
6300.0
Implemented on
2002-11-15
Delivery
via email in 1~10 business day
Detail of XDJSGF2022
Standard No.
XDJSGF2022
English Name
Technical Specification for Disinfection, 2002
Chinese Name
消毒技术规范，2002年版
Chinese Classification
Professional Classification
XD
ICS Classification
Issued by
Issued on
2002-11-15
Implemented on
2002-11-15
Status
valid
Superseded by
Superseded on
Abolished on
Superseding
Language
English
File Format
PDF
Word Count
130,000 words
Price(USD)
6300.0
Keywords
XDJSGF2022, , XDJSGF2022,
Introduction of XDJSGF2022
2.1.3 Identification test of air disinfection efficacy
2.1.3.1 Purpose
Detect the killing and/or cleaning effect of disinfecting apparatus and disinfectant on bacteria in the air in order to verify its disinfection efficacy to air. For the purposes of the disinfection efficacy of other methods to air, the relevant principles of this test may also be referred to.
2.1.3.2 Test equipment and apparatus
(1) The test bacterium is Staphylococcus albus 8032, and see 2.1.1.2 for the preparation method of the bacterial suspension.
(2) Sampling liquid [used for sampling with liquid-impacting sampler. During sterilization test using non-chemical factor, nutrient broth medium containing anti-foaming agent (octanol or olive oil) shall be used; during sterilization test using disinfectant, nutrient broth medium containing corresponding neutralizer (octanol or olive oil also added) shall be used].
(3) Neutralizer (qualified upon identification according to the method specified in 2.1.1.5).
(4) Phosphate buffer (PBS, 0.03 mol/L, pH 7.2).
(5) Common nutrient broth medium.
(6) Common nutrient agar medium. In the sterilization test using disinfectant, corresponding neutralizer need to be added in it.
(7) A pair of adjacent aerosol cabinets or chambers, one for disinfection test and the other for test control. The environment in which a pair of adjacent aerosol cabinets or chambers are located shall be the same (including temperature, humidity, lighting, airtightness and ventilation conditions). Cabinets (or chambers) should be constructed of aluminum alloy and glass. Temperature and humidity regulating devices, fan-filtering sterilization or other disinfection devices together with corresponding pipelines shall be installed. In addition, windows for spraying for bacteria contamination, operating sleeves for disinfectant supplying and sampling and transferring sample shall be opened.
(8) Spraying device for bacteria contamination, including air compressor, pressure gauge, gas flowmeter, aerosol sprayer, etc. More than 90% of bacterial aerosol particles shall have a diameter of 1μm~10μm.
(9) Air microorganism sampling device, including six-stage mesh air-impacting sampler, liquid-impacting sampler, air extraction equipment, gas flowmeter, etc.
(10) Environmental monitoring equipment, such as thermometers and hygrometers.
2.1.3.3 Test stage
Air disinfection tests are divided into laboratory tests, simulated field tests and field tests. The characteristics of the tests in three stages are shown in Table 2-5.
2.1.3.4 Operating procedures for laboratory tests and simulated field tests
(1) Take the bacterial suspension of test bacteria, filter it with sterile absorbent cotton, and dilute it with nutrient broth medium to the required concentration.
(2) Simultaneously adjust the temperature and relative humidity of the two aerosol cabinets (or chambers) to the required ones for the test.
(3) Put the equipment used into the aerosol cabinets (or chambers) at one time and close the doors. After that, all operations and operation of instruments and equipment are carried out outside the cabinets (or chambers) through windows with sealed sleeves or through remote controllers. The door may not be opened until the test is finished.
(4) Spray for bacteria contamination according to the set pressure, gas flow and spray time. Spray the contaminated bacteria while stirring with a fan. After the completion of spraying contaminated bacteria, continue to stir for 5min, and then let stand for 5min.
(5) At the same time, conduct sampling for the aerosol cabinets (chambers) of the control group and the test group before disinfection, respectively, as positive controls (i.e. the amount of contaminated bacteria) before conducting the test of the control group and the disinfection treatment of the test group. As for the air in the aerosol cabinets (chambers), the number of bacteria used as positive control shall reach 5´104cfu/m3~5´106cfu/m3.
Table 2-5 Characteristics of air disinfection tests at each stage
