Standard No.:	GB 29989-2013
English Name:	Determination of L-carnitine in infant foods and dairy products
Chinese Name:	婴幼儿食品和乳品中左旋肉碱的测定
Chinese Classification:	X09    Hygiene, safety and labor protection
Professional Classification:	GB    National Standard
Source Content Issued by:	Health and Family Planning Commission
Issued on:	2013-11-29
Implemented on:	2014-6-1
Status:	superseded
Superseded by:	GB 5009.300-2025 National food safety standard - Determination of L-carnitine in the foods
Superseded on:	2025-9-16
Abolished on:	2025-09-16
Target Language:	English
File Format:	PDF
Word Count:	1500 words
Translation Price(USD):	55.0
Delivery:	via email in 1 business day

1 Scope This standard specifies the method for determination of levocarnitine in foods and milk products for infants and young children. This standard is applicable to the determination of levocarnitine in foods and milk products for infants and young children. 2 Principle Extract the specimen with water and make the protein to sedimentate with perchloric acid prior to filtration. After alkaline saponification, the levocarnitine in combined state is dissociated from the filtrate. Under the catalysis of carnitine acetyl transferase, levocarnitine reacts with acetyl coenzyme A to generate acetylcarnitine and free coenzyme A. Free coenzyme A reacts with 2-nitrobenzoic acid to generate yellow substance, the color of which is proportional to the content of free coenzyme A. As the free coenzyme A and levocarnitine are of same mole reaction, the content of levocarnitine in specimen may be calculated indirectly. 3 Reagents and Materials Note: unless otherwise specified, all reagents adopted in this method are analytical reagents and the water is Grade 3 specified in GB/T 6682.

1 Scope 2 Principle 3 Reagents and Materials 4 Apparatus and Equipment 5 Analytical Procedures 6 Expression of analytical result 7 Precision 8 Other