Standard No.:	GB 5009.189-2023
English Name:	National food safety standard - Determination of bongkrekic acid in foods
Chinese Name:	食品安全国家标准 食品中米酵菌酸的测定
Chinese Classification:	X09    Hygiene, safety and labor protection
Professional Classification:	GB    National Standard
Source Content Issued by:	NHC; SAMR
Issued on:	2023-9-6
Implemented on:	2024-9-6
Status:	valid
Superseding:	GB 5009.189-2016 Determination of bongkrekic acid in tremella fuciformis berk
Target Language:	English
File Format:	PDF
Word Count:	7500 words
Translation Price(USD):	225.0
Delivery:	via email in 1 business day

GB 5009.189-2023 National food safety standard - Determination of bongkrekic acid in foods 1 Scope This standard specifies the method for determination of bongkrekic acid in foods. The Method I of this standard is applicable to the determination of bongkrekic acid in tremella and its products, fermentive corn flour and its products. The Method II of this standard is applicable to the determination of bongkrekic acid in tremella and its products, auricularia auricular and its products, as well as cereals and their products. Method I Liquid chromatography 2 Principle Extract bongkrekic acid in the specimens with solvent, then purify and concentrate it by mixed strong anion exchange column or liquid-liquid extraction method, analyse with high performance liquid chromatograph, and quantify by external standard method. 3 Reagents and materials Unless otherwise stated, reagents used in this method are analytical pure, and the water is Class-I water as specified in GB/T 6682. 3.1 Reagents 3.1.1 Methanol (CH3OH): chromatographically pure. 3.1.2 Methanol (CH3OH). 3.1.3 Ammonia water (NH3·H2O): 25%~28%. 3.1.4 Formic acid (CH2O2). 3.1.5 Hydrochloric acid (HCl). 3.1.6 Phosphoric acid (H3PO4). 3.1.7 Sodium bicarbonate (NaHCO3). 3.1.8 Petroleum ether (C5H12O2): with a boiling range of 30℃~60℃. 3.1.9 Ethyl ether absolute (C4H10O) 3.1.10 Trichloromethane (CHCl3). 3.2 Reagent preparation 3.2.1 Phosphoric acid solution (45.4%): measure 45.4mL of phosphoric acid and put it into a 100mL volumetric flask, dilute it to the scale with water and shake well.

Foreword i 1 Scope 2 Principle 3 Reagents and materials 4 Instruments and apparatus 5 Analytical procedure 6 Calculation and expression of results 7 Precision 8 Others 9 Principle 10 Reagents and materials 11 Instruments and apparatus 12 Analytical procedure 13 Calculation and expression of results 14 Precision 15 Others Annex A High performance liquid chromatogram of bongkrekic acid standard solution Annex B MRM mass chromatogram of bongkrekic acid standard solution