T/CIFST 006-2021 Detection of lactoferrin in foods — Enzyme-linked immunosorbent assay method
1 Scope
This document specifies an enzyme-linked immunosorbent assay method for the determination of lactoferrin in food.
This document is applicable to the determination of lactoferrin content in raw milk, pasteurized milk, mixed milk, flavored fermented milk, milk containing beverages and other liquid foods containing milk or added milk ingredients, as well as solid foods containing milk or added milk ingredients, such as mixed milk powder, infant formula milk powder and solid beverages.
2 Normative references
The following documents contain provisions which, through reference in this text, constitute provisions of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682-2008 Water for analytical laboratory use - Specification and test methods
3 Terms and definitions
No terms and definitions are listed in this document.
4 Principle
The lactoferrin content in samples can be determined by competitive enzyme-linked immunosorbent assay method using specific antigen-antibody binding reaction.
Free lactoferrin in a standard or sample can bind at the binding site with the lactoferrin antibody pre-coated in the competitive binding micropores of its enzyme marker. Unbound lactoferrin shall be removed during the washing step. The colorless substrate can be converted, by bound enzyme marker, into a blue product. The color changed from blue to yellow when the reaction termination solution is added. The lactoferrin content in the standard or sample is inversely proportional to the absorbance value. By measuring the absorbance value at 450nm wavelength and drawing the standard curve, the lactoferrin content in the sample can be calculated.
Foreword II
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
6 Apparatus
7 Analytical procedures
8 Result and calculation
9 Quality control
10 Limits of detection and quantification
Annex A (Nominative) Test kit for determination of lactoferrin by enzyme-linked immunosorbent assay method
T/CIFST 006-2021 Detection of lactoferrin in foods — Enzyme-linked immunosorbent assay method
1 Scope
This document specifies an enzyme-linked immunosorbent assay method for the determination of lactoferrin in food.
This document is applicable to the determination of lactoferrin content in raw milk, pasteurized milk, mixed milk, flavored fermented milk, milk containing beverages and other liquid foods containing milk or added milk ingredients, as well as solid foods containing milk or added milk ingredients, such as mixed milk powder, infant formula milk powder and solid beverages.
2 Normative references
The following documents contain provisions which, through reference in this text, constitute provisions of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6682-2008 Water for analytical laboratory use - Specification and test methods
3 Terms and definitions
No terms and definitions are listed in this document.
4 Principle
The lactoferrin content in samples can be determined by competitive enzyme-linked immunosorbent assay method using specific antigen-antibody binding reaction.
Free lactoferrin in a standard or sample can bind at the binding site with the lactoferrin antibody pre-coated in the competitive binding micropores of its enzyme marker. Unbound lactoferrin shall be removed during the washing step. The colorless substrate can be converted, by bound enzyme marker, into a blue product. The color changed from blue to yellow when the reaction termination solution is added. The lactoferrin content in the standard or sample is inversely proportional to the absorbance value. By measuring the absorbance value at 450nm wavelength and drawing the standard curve, the lactoferrin content in the sample can be calculated.
Contents of T/CIFST 006-2021
Foreword II
1 Scope
2 Normative references
3 Terms and definitions
4 Principle
5 Reagents and materials
6 Apparatus
7 Analytical procedures
8 Result and calculation
9 Quality control
10 Limits of detection and quantification
Annex A (Nominative) Test kit for determination of lactoferrin by enzyme-linked immunosorbent assay method
