GB/T 30636-2014 Determination of sialic acid in swiflets nest and swiflets nest products―Liquid chromatographic method English
1 Scope
This standard specifies the liquid chromatographic method for determining sialic acid in swiflets nest and swiflets nest products.
The standard is applicable to determining sialic acid in swiflets nest and swiflets nest products.
The limit of detection of the method in this standard is 0.3g/kg for swiflets nest and 0.003g/kg for swiflets nest products.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
GB/T 6682 Water for analytical laboratory use - Specification and test methods
3 Method summary
Swiflets nests and swiflets nest products are heated and hydrolyzed in acetic acid solution to release sialic acid. The hydrolysate is separated by strong cation exchange column, detected by liquid chromatography - ultraviolet detector and quantified by external standard method.
4 Reagents and materials
4.1 Water: meeting the requirements of Class-I water specified in GB/T 6682.
Foreword II 1 Scope 2 Normative references 3 Method summary 4 Reagents and materials 5 Apparatuses 6 Analytical steps 7 Determination 8 Result calculation 9 Precision Annex A (Informative) Chromatograms of sialic acid standard solutions and swiflets nest specimen hydrolysates
GB/T 30636-2014 Determination of sialic acid in swiflets nest and swiflets nest products―Liquid chromatographic method English
1 Scope
This standard specifies the liquid chromatographic method for determining sialic acid in swiflets nest and swiflets nest products.
The standard is applicable to determining sialic acid in swiflets nest and swiflets nest products.
The limit of detection of the method in this standard is 0.3g/kg for swiflets nest and 0.003g/kg for swiflets nest products.
2 Normative references
The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.
GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method
GB/T 6682 Water for analytical laboratory use - Specification and test methods
3 Method summary
Swiflets nests and swiflets nest products are heated and hydrolyzed in acetic acid solution to release sialic acid. The hydrolysate is separated by strong cation exchange column, detected by liquid chromatography - ultraviolet detector and quantified by external standard method.
4 Reagents and materials
4.1 Water: meeting the requirements of Class-I water specified in GB/T 6682.
Contents of GB/T 30636-2014
Foreword II
1 Scope
2 Normative references
3 Method summary
4 Reagents and materials
5 Apparatuses
6 Analytical steps
7 Determination
8 Result calculation
9 Precision
Annex A (Informative) Chromatograms of sialic acid standard solutions and swiflets nest specimen hydrolysates
