Foreword
Codeofchina.com is in charge of this English translation. In case of any doubt about the English translation, the Chinese original shall be considered authoritative.
This standard is the substitute of GB/T 4789.2-2008 "Microbiological Examination of Food Hygiene-Aerobic Plate Count".
The main amendments of this standard comparing with GB/T 4789.5-2008 are as follows:
——The Chinese and English names of this standard were amended;
——The explanation in aerobic plate count calculation formula was amended;
——Culture medium and reagent were amended;
——The second method: PetrifilmTM aerobic plate count method was deleted.
The annex A of this standard is normative.
The issue condition of all previous editions of standards substituted by this standard is:
——GB 4789.2-1984, GB 4789.2-1994, GB/T 4789.2-2003, GB/T 4789.2-2008.
National Food Safety Standard
Food Microbiological Examination: Aerobic Plate Count
食品安全国家标准
食品微生物学检验 菌落总数测定
1 Scope
This standard specifies examination method of aerobic plate count in food.
This standard is applicable to the examination of aerobic plate count in food.
2 Terms and Definitions
2.1 Aerobic plate count
It refers to the total microbes colonies amount in per g or mL of specimen after food specimen has been treated and cultured under certain conditions (such as culture medium, culture temperature and incubation time).
3 Equipments and Materials
Except conventional sterilization in microbiology Lab. and culture equipments, other equipments and materials are as follows:
3.1 Thermostatic incubator36℃±1℃, 30℃±1℃.
3.2 Refrigerator: 2℃~5℃.
3.3 Thermostatic water tank: 46±1℃.
3.4 Scale: sensibility is 0.1 g.
3.5 Homogenizer.
3.6 Oscillator.
3.7 Sterile suckers: 1mL (with 0.01mL scale), 10mL (with 0.1 scales) or micropipette and suction head.
3.8 Sterile conical flasks: capacities are 250mL and 500mL.
3.9 Sterile culture dish: diameter is 90mm.
3.10 Acidimeter, pH color comparison tube or short-range pH paper.
3.11 Magnifier or/and colony counter.
4 Culture Medium and Reagents
4.1 Plate count agar culture medium: see A.1 in Annex A.
4.2 Phosphate buffer: see A.2 in Annex A.
4.3 Sterile physiological saline: see A.3 in Annex A.
Foreword
1 Scope
2 Terms and Definitions
3 Equipments and Materials
4 Culture Medium and Reagents
5 Examination Procedures
6 Operation Steps
7 Results and Reports
Annex A (Normative) Culture Medium and Reagent
Foreword
Codeofchina.com is in charge of this English translation. In case of any doubt about the English translation, the Chinese original shall be considered authoritative.
This standard is the substitute of GB/T 4789.2-2008 "Microbiological Examination of Food Hygiene-Aerobic Plate Count".
The main amendments of this standard comparing with GB/T 4789.5-2008 are as follows:
——The Chinese and English names of this standard were amended;
——The explanation in aerobic plate count calculation formula was amended;
——Culture medium and reagent were amended;
——The second method: PetrifilmTM aerobic plate count method was deleted.
The annex A of this standard is normative.
The issue condition of all previous editions of standards substituted by this standard is:
——GB 4789.2-1984, GB 4789.2-1994, GB/T 4789.2-2003, GB/T 4789.2-2008.
National Food Safety Standard
Food Microbiological Examination: Aerobic Plate Count
食品安全国家标准
食品微生物学检验 菌落总数测定
1 Scope
This standard specifies examination method of aerobic plate count in food.
This standard is applicable to the examination of aerobic plate count in food.
2 Terms and Definitions
2.1 Aerobic plate count
It refers to the total microbes colonies amount in per g or mL of specimen after food specimen has been treated and cultured under certain conditions (such as culture medium, culture temperature and incubation time).
3 Equipments and Materials
Except conventional sterilization in microbiology Lab. and culture equipments, other equipments and materials are as follows:
3.1 Thermostatic incubator36℃±1℃, 30℃±1℃.
3.2 Refrigerator: 2℃~5℃.
3.3 Thermostatic water tank: 46±1℃.
3.4 Scale: sensibility is 0.1 g.
3.5 Homogenizer.
3.6 Oscillator.
3.7 Sterile suckers: 1mL (with 0.01mL scale), 10mL (with 0.1 scales) or micropipette and suction head.
3.8 Sterile conical flasks: capacities are 250mL and 500mL.
3.9 Sterile culture dish: diameter is 90mm.
3.10 Acidimeter, pH color comparison tube or short-range pH paper.
3.11 Magnifier or/and colony counter.
4 Culture Medium and Reagents
4.1 Plate count agar culture medium: see A.1 in Annex A.
4.2 Phosphate buffer: see A.2 in Annex A.
4.3 Sterile physiological saline: see A.3 in Annex A.
Contents of GB 4789.2-2010
Foreword
1 Scope
2 Terms and Definitions
3 Equipments and Materials
4 Culture Medium and Reagents
5 Examination Procedures
6 Operation Steps
7 Results and Reports
Annex A (Normative) Culture Medium and Reagent
